医学英语分子生物学实验,英译汉
ComparethecDNAsequenceof4variants,thereareonlydifferencesbetweenexon3andexon7,thusdesignspecificprimerforthesetwoexonscoulddifferentiate4variants.
1.totalRNAextraction
2.RT-PCR:
primerforexon3:Tm:about52°C
senseprimer:5'GATTGGACAGGCTGGACT3'
anti-senseprimer:5'AGAAGTGGTGGCAGTGATT3'
predictedresults:288bpproductinNurr1-a,b,c;noamplifiedproductinNurr1-d.
primerforexon7:Tm:58-59°C
senseprimer:5'CCAAAGCCGACCAAGACC3'
anti-senseprimer:5'ATTATTGCTGGCGGTGGC3'
predictedresults:472bpproductinNurr1-a,d;418bpproductinNurr1-b;351bpproductinNurr1-c.
3.resultanalysis:
Nurr1-aNurr1-bNurr1-cNurr1-d
amplicationforexon3288bp288bp288bpnoproduct
amplicationforexon7472bp418bp351bp472bp
4.
Problem:RefSeqforNurr1-b,c,dhavebeenpermanentlysuppressedbecauseofinsufficientsupport.
