问题标题:
分子生物学文章求翻译NIPP1isaubiquitousnuclearproteinthatisrequiredforspliceosomeassembly.Wereportherethatthephos-phothreonine-bindingForkhead-associateddomainofNIPP1interactswiththecellcycle-regulatedproteinSer/Thr
问题描述:
分子生物学文章求翻译
NIPP1isaubiquitousnuclearproteinthatisrequired
forspliceosomeassembly.Wereportherethatthephos-
phothreonine-bindingForkhead-associateddomainof
NIPP1interactswiththecellcycle-regulatedprotein
Ser/ThrkinaseMELK(maternalembryonicleucinezip-
perkinase).TheNIPP1-MELKinteractionwascritically
dependentonthephosphorylatonofThr-478ofMELK
andwasincreasedinlysatesfrommitoticallyarrested
cells.RecombinantMELKwasapotentinhibitorofan
earlystepofspliceosomeassemblyinnuclearextracts.
Thissplicingdefectwasalsoseenwithakinase-dead
mutantbutwasabsentaftermutation(T478A)ofthe
NIPP1bindingsiteofMELK,indicatingamediatory
roleforNIPP1.OurdatasuggestthatMELKhasarolein
thecellcycle-regulatedcontrolofpre-mRNAsplicing.
侯锡九回答:
NIPP1是一种普遍存在于细胞核中的蛋白,它在剪接体的装配中必须是的.在这里,我们报道NIPP1中与磷酸化苏氨酸结合的forkhead相关结构域和细胞周期调控蛋白——Ser/Thr激酶MELK之间的相互作用.NIPP1与MELK之间的作用严格...
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